Introduction: Ocular trauma is defined as a trauma caused by blunt or penetrating mechanisms on the eyeball\nand its peripheral structures, causing damage with different degrees of affection with temporary or permanent\nvisual function compromise. Ocular trauma is a major cause of preventable blindness worldwide; it constitutes 7%\nof all corporal injury and 10% to 15% of all eye diseases. Regenerative medicine research has opened up the\npossibility to use stem cells as a source of cell replacement, so that experimental studies on embryonic stem cells\nand bone marrow stem cells have been carried out. In this study, we analyzed the histopathological and\nspectroscopic changes in ocular tissue with trauma, treated with mouse pluripotent stem cells.\nMethods: Firstly, mouse embryonic stem cells were seeded. Subsequently, the obtained cells were implanted in a\nmurine model of scleral and retinal damage at the first, second, and fourth weeks post-trauma. At week 12 post-trauma,\nthe eyes were enucleated for histopathologic study (inflammatory response and histological integrity) and spectroscopic\nanalysis by Fourier transform infrared spectroscopy in the attenuated total reflection configuration. Data were analyzed\nby one-way analysis of variance.\nResults: Histopathological results showed that the experimental groups treated with stem cells presented a decrease in\nthe inflammatory response, and the histological integrity was restored, which contrasted with the experimental group\ntreated with saline solution. Moreover, in the spectroscopic analysis, characteristic bands of biological samples were\nobserved in all tissues, highlighting in healthy tissues the presence of C = O bond at 1,745 cm-1, which was not\nobserved in the injured and treated tissues. Also, the absorption spectrum of the tissues treated with embryonic stem\ncells showed bands whose intensity was high at around 1,080 to 1,070 cm-1. It has been reported that these bands are\ncharacteristic of pluripotent stem cells.\nConclusions: The implant of embryonic stem cells could be a useful therapeutic treatment after traumatic eye injuries\nor many other eye diseases to reduce the inflammatory response and restore histological integrity. Furthermore, the\nspectroscopic technique could be used as a complementary technique for detecting stem cell incorporation into\nvarious tissues
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